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探讨缺失羟基丙酮酸还原酶基因对莱茵衣藻光合能力及物质积累的影响。本文以模式生物莱茵衣藻（Chlamydomonas reinhardtii）为对象，通过比较野生型UVM4与突变株Crhgm1在不同生长阶段的生理特性，揭示线粒体NADH依赖性羟基丙酮酸还原酶CrHPR1(hydroxypyruvate reductase 1 in Chlamydomonas reinhardtii)缺失产生的影响。研究结果表明与光自养条件相比，兼性培养促进藻细胞生长，明显缩短其生长周期；CrHPR1基因缺失导致藻株Fv/Fm和NPQ分别提高19.37%和27.89%，表明光合效率和光保护能力增强，但对细胞的保护能力减弱；突变株Crhgm1生物合成能力增强，总叶绿素、蛋白质、可溶性糖和油脂含量分别较野生型提高16%～27%，其中平台期油脂积累达干重的56.49%。CrHPR1基因缺失对莱茵衣藻光合效率、光保护能力及代谢物积累的积极影响，尤其在油脂积累方面展现出应用潜力。本研究为微藻的高值化利用提供了新的思路，并具有在生物能源开发中的应用潜力。
Abstract：Exploring the effects of missing hydroxypyruvate reductase gene on the photosynthetic capacity and substance accumulation of Chlamydomonas reinhardtii.The model organism Chlamydomonas reinhardtii was used to investigate the physiological characteristics of the wild-type UVM4 and the mutant strain Crhgm1 at different growth stages, revealing the effects of mitochondrial NADH-dependent hydroxypyruvate reductase CrHPR1 gene knockout. The results show that: mixotrophic cultivation significantly enhanced cellular growth rates and reduced cultivation duration relative to photoautotrophic conditions; the CrHPR1 gene knockout led to a 19.37% increase in Fv/Fm and a 27.89% increase in NPQ, indicating enhanced photosynthetic efficiency and photoprotective capacity, although the protective effect on cells was weakened; the Crhgm1 mutant exhibited enhanced biosynthetic capacity, with total chlorophyll, protein, soluble sugar, and lipid content increasing by 16%～27% compared to the wild type, with lipid accumulation reaching 56.49% of dry weight during the stationary phase. The deletion of the CrHPR1 gene has a positive impact on the photosynthetic efficiency, photoprotection ability, and metabolite accumulation of Chlamydomonas reinhardtii, especially in terms of oil accumulation, demonstrating potential applications.These findings provide new insights for the high-value utilization of microalgae and hold significant potential for applications in bioenergy development.
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基本信息:
中图分类号:Q945
引用信息:
[1]路思晗,罗晨,邓必成,等.缺失羟基丙酮酸还原酶基因对莱茵衣藻光合能力及物质积累的影响[J].华北理工大学学报(自然科学版),2025,47(04):21-30.
基金信息:
国家重点研发计划（2022YFC3401800）; 唐山科技计划（24150214C）